1. Theta, Union College (1833, inactive since 2014)

2. Delta, New York University (1837)

3. Beta, Yale University (1839, inactive since 1934)

4. Sigma, Brown University (1840, inactive since 1993)

5. Gamma, Amherst College (1841, inactive since 2010) 

6. Zeta, Dartmouth College (1842)

7. Lambda, Columbia University (1842, inactive since 2014)

8. Kappa, Bowdoin College (1843, inactive since 1998)

9. Psi, Hamilton College (1843) 

10. Xi, Wesleyan University (1843)

11. Alpha, Harvard University (1850, inactive since 1873)

12. Upsilon, University of Rochester (1858)

13. Iota, Kenyon College (1860, inactive since 2010)

14. Phi, University of Michigan (1865) 

15. Omega, University of Chicago (1869)

16. Pi, Syracuse University (1875)

17. Chi, Cornell University (1876, suspended 2016)

18. Beta Beta, Trinity College (1880)

19. Eta, Lehigh University (1884) 

20. Tau, University of Pennsylvania (1891)

21. Mu, University of Minnesota (1891, inactive since 1993)

22. Rho, University of Wisconsin (1896, inactive since 2021)

23. Epsilon, University of California, Berkeley (1902, inactive since 1998)

24. Omicron, University of Illinois (1910)

25. Delta Delta, Williams College (1913, inactive since 1968) 

26. Theta Theta, University of Washington (1916)

27. Nu, University of Toronto (1920, inactive since 1997)

28. Epsilon Phi, McGill University (1928, inactive since 1997)

29. Zeta Zeta, University of British Columbia (1935)

30. Epsilon Nu, Michigan State University (1943) 

31. Epsilon Omega, Northwestern University (1949, inactive since 1999)

32. Theta Epsilon, University of Southern California (1950, inactive since 1962)

33. Nu Alpha, Washington and Lee University (1970, inactive since 1974)

34. Gamma Tau, Georgia Institute of Technology (1970) 

35. Chi Delta, Duke University (1973)

36. Zeta Tau, Tufts University (1981, inactive 1992)

37. Epsilon Iota, Rensselaer Polytechnic Institute (1982)

38. Phi Beta, College of William and Mary (1984, Owl Club 2020)

39. Kappa Phi, Pennsylvania State University (1989, inactive since 1998) 

40. Beta Kappa, Washington State University (1991, inactive since 2003)

41. Beta Alpha, Miami University of Ohio (1992, inactive since 1996)

42. Phi Delta, University of Mary Washington (1996)

43. Lambda Sigma, Pepperdine University (1998)

44. Alpha Omicron, New Jersey Inst. of Technology (1999) 

45. Sigma Phi, St. Francis University (2007)

46. Delta Nu, Keene State College (2009, inactive since 2020)

47. Phi Nu, Christopher Newport University (2010, suspended 2023)

48. Theta Pi, Georgia State University (2014, inactive since 2015)

49. Tau Epsilon, Clemson University (2018)

50. Delta Omicron Provisional Chapter, Purdue University